Final Lab

Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity, in this case

the ability of the enzyme catalase to break down hydrogen peroxide. (Recall: Enzymes are proteins.) If
necessary, it may be useful for you to review the Scientific Method Tutorial (found in the Course Content
section of the classroom).

Lab Materials

You may need the following, depending on your experimental design:

plastic beakers or cups
hydrogen peroxide solution
vinegar to provide an acid liquid, 1 part vinegar to 20 parts tap water has pH of about 3
sample of fresh meat, about 1 cm cubed in size (unprocessed and uncooked, e.g., liver, steak, fish, or
poultry—particularly organs)
sample of fresh vegetable, about 1 cm cubed in size (e.g., potatoes or other root vegetables)
a pen for labeling the beakers or cups
a ruler with centimeter markings
1. What question are you asking? Clearly state your hypothesis.

2. Design an experiment. Provide a detailed account of the materials and methods used to conduct the
experiment. Also include the methods for data collection and analysis. Remember that an a good experiment
needs control and experimental groups.

3. Conduct the experiment and record your results below. What did you observe? Which samples showed bubbling?

4. Use your knowledge about enzymes to interpret your results. It may be necessary for you to refer to your
textbook. What effect does the acid have on the enzyme?

5. Was your hypothesis supported? What is your conclusion?
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