The method of collection of immature oocytes can affect mitochondrial morphology and function : Follicle containing immature oocytes can be collected from ovaroan tissue by Mechanical disaggregation (use forceps and seisors 2. Enzyme digestion of the extra cellular matrix and connective tissue that the follicles embedded in - collagenase - DNAse - Libarase - tryse 3. A combination of mitochondrial and enzyme disagrregation Collect ovaries from naturally cycling mice culled in routine anthiel hwse procedures 2. Dissect into rephiate small sections 3. Distribute to 4% paraformaldehyde fixation H+E histology to quantity follicule Mechanical disaggregation Collagenase concentration 1, 2 & 3 Trypsin concentration 1, 2 & 3 Selected cllagenase concentration + selected hypsin concentration sTo determine optomum time for each incubation Place an order with similar or related instructions NOW

This is the practical infomation. Investigation of Transcriptional Control of Genes INTRODUCTION Nitrate is assimilated in the leaves of higher plants by the enzymes nitrate reductase and nitrite reductase, the ammonia produced is incorporated into amino acids. METHOD You have been provided with trays of pea seedlings which have been grown for 14 days in the light in the absence of NO3-. 24 hours prior to the experiment they have been treated as follows: Kept in Light Transferred to Dark 1 +NO3(14mM) 3 +NO3(14mM) 2 -NO3 4 -NO3 Remove approximately 0.5g of leaf tissue from each treatment (using approximately the same age of leaves where possible) and weigh accurately. Keep the leaves cold on watch glasses in an ice bucket, and slice into thin (1-2mm) sections with razor blade.Transfer the tissue to an ice-cooled side arm tube and add 5ml of the following chilled reaction medium, pH 7.5, containing: 50 mm KH2PO4 100mm KNO3 100mm KCH3CO2 (potassium acetate) 1.5% v/v propan-1-ol Atta