Laboratory Techniques
Laboratory Techniques
1. What results should you obtain on each of the plates? Give an indication of the number of colonies that you would expect to see on each plate, whether the bacteria should glow (express GFP) and also for each plate explain why the results should be as you have described.
a) +pGlo LB/amp
b) +pGlo LB/amp/ara
c) -pGlo LB
d) -pGlo LB/amp
2. What is meant by control plate? Which plates in your experiment were controls and what purpose does a control(s) serve?
3 Explain in detail the purpose of the arabinose in the LB media and how it causes its effect on the transformed E. coli?
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